Team:Paris Saclay/Project/Odor-free ecoli

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The ''E. coli'' odor free chassis===
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The ''E. coli'' odor free chassis
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''Escherichia coli'' stinks because of the ''tnaA'' gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene.
''Escherichia coli'' stinks because of the ''tnaA'' gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene.
[[File:IndoleFormation.jpg|500px|center]]
[[File:IndoleFormation.jpg|500px|center]]

Revision as of 10:02, 6 October 2014

Countdown

This page is under Romain's responsibility

  • Deadline: 08/oct.
    • Completed text.
  • Deadline: 12/oct
    • Final review Philipe.

Odor-free E.coli chassis

The E. coli Odor Free Chassis

The E. coli odor free chassis

Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene.

IndoleFormation.jpg

In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but this strain was too modified to be used for our project. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all.

OdorFree.jpg