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Team:Paris Saclay/Project/Odor-free ecoli
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=Odor-free E.coli chassis= | =Odor-free E.coli chassis= | ||
| - | == | + | ==The E. coli Odor Free Chassis== |
| - | + | The ''E. coli'' odor free chassis=== | |
| + | ''Escherichia coli'' stinks because of the ''tnaA'' gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. | ||
| + | [[File:IndoleFormation.jpg|500px|center]] | ||
| + | In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but this strain was too modified to be used for our project. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all. | ||
| - | + | [[File:OdorFree.jpg|500px|center]] | |
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Revision as of 16:17, 2 October 2014
Odor-free E.coli chassis
The E. coli Odor Free Chassis
The E. coli odor free chassis=== Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene.
In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but this strain was too modified to be used for our project. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all.
