Team:Paris Saclay/Notebook/September/15

From 2014.igem.org

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(B-B - Construction of the fusion protein (color))
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==Lab Work==
==Lab Work==
''by Mélanie''
''by Mélanie''
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====transformation for stock of the w6 clone====
====transformation for stock of the w6 clone====
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Revision as of 19:23, 29 September 2014

Contents

Lab Work

by Mélanie

D-lemon scent

Transformation with the ligation (futur pPS2/4)

liquide culture of some PS clone (topo)

Transformation of LS GS CAS (topo) to do stock

PCR pSCBC3 cloning

PCR of GS : primer with ecoRI and PSTI restriction site

B- Construction of the fusion protein (color)

PCR

We have decided to separate the two protein So I do a PCR to have the yellow and the blue protein and i cloned them in a topo plasmid.

transformation for stock of the w6 clone