Team:Paris Saclay/Notebook/September/15


(Difference between revisions)
(D-lemon scent)
Line 3: Line 3:
''by Mélanie''
''by Mélanie''
===D-lemon scent===
===lemon scent===
====Transformation with the ligation (futur pPS2/4)====
====Transformation with the ligation (futur pPS2/4)====

Revision as of 09:17, 14 October 2014


Lab Work

by Mélanie

lemon scent

Transformation with the ligation (futur pPS2/4)

liquide culture of some PS clone (topo)

Transformation of LS GS CAS (topo) to do stock

PCR pSCBC3 cloning

PCR of GS : primer with ecoRI and PSTI restriction site

B- Construction of the fusion protein (color)


We have decided to separate the two protein So I do a PCR to have the yellow and the blue protein and i cloned them in a topo plasmid.

transformation for stock of the w6 clone

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