Team:Paris Saclay/Notebook/September/10

From 2014.igem.org

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=Wednesday 10th September=
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==Lab Work==
==Lab Work==
''by Mélanie''
''by Mélanie''
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===D-Lemon Scent===
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===Lemon Scent===
====Plasmid extraction====
====Plasmid extraction====
From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/9#PCR Yesterday] I do a plasmid extraction.
From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/9#PCR Yesterday] I do a plasmid extraction.
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[[File:1009 vérif extraction plasmides.jpg|300px]]
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[[File:1009 vérif extraction plasmides.jpg|300px|center]]
these clones (LS GS CAD) had sent to sequencing
these clones (LS GS CAD) had sent to sequencing
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[[File:1009 digestion topo pacI.jpg|200PX]]
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[[File:1009 digestion topo pacI.jpg|200PX|center]]
====Purification====
====Purification====
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* ''Translecture system'' : maybe we have to give up this part of our work because of the new project.
* ''Translecture system'' : maybe we have to give up this part of our work because of the new project.
* ''Diffusion of the limonene'' : Eugène has focused on the concentration of limonene in the atmosphere.
* ''Diffusion of the limonene'' : Eugène has focused on the concentration of limonene in the atmosphere.
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{{Team:Paris_Saclay/default_footer}}
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==Photo of the Day==
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[[File:Paris Saclay 10_september.jpg|600px|center]]
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[http://emmanuelhirsch.fr/ Emmanuel Hirsch] , one of those few we have interviewed during this month of september.
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To learn more about the ethic aspect of our project, just click [https://2014.igem.org/Team:Paris_Saclay/Ethics here]
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 09:15, 14 October 2014

Contents

Wednesday 10th September

Lab Work

by Mélanie

Lemon Scent

Plasmid extraction

From the liquide culture made Yesterday I do a plasmid extraction.

1009 vérif extraction plasmides.jpg

these clones (LS GS CAD) had sent to sequencing problem with ps

digestion

I digest with PacI the plasmid that contain LS and GS

component volume
H2O 5μl
buffer 3μl
PacI 1μl
Plasmid 20μl
200PX

Purification

Electrophoresis to isolate the insert and purification with a kit = I failed

culture

As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B

Modeling

by Arnaud, Eugène, Juliette and Pierre

Meeting about modeling with advisor Claire to talk about the achievement of our model

  • Diffusion of O2 in the gel : maths is OK but biologist need more drawings.
  • Bacterial growth on the surface : because of the new aspect of our project, we have to change our point of view. Tomorrow, a real discussion with the biologists is needed to understand more precisely what they are doing in the lab.
  • Translecture system : maybe we have to give up this part of our work because of the new project.
  • Diffusion of the limonene : Eugène has focused on the concentration of limonene in the atmosphere.

Photo of the Day

Paris Saclay 10 september.jpg

[http://emmanuelhirsch.fr/ Emmanuel Hirsch] , one of those few we have interviewed during this month of september.

To learn more about the ethic aspect of our project, just click here


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