Team:Paris Saclay/Notebook/September/1

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(Created page with "==B-Construction of the fusion protein== 'by Hoang Vu' We pricked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri di...")
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==B-Construction of the fusion protein==
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{{Team:Paris_Saclay/notebook_header}}
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=Monday 1st September=
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==Lab Work==
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===Construction of the fusion protein===
'by Hoang Vu'
'by Hoang Vu'
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We pricked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent prick out.
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We picked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent pick out.
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===Lemon scent===
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'' by melanie''
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====Limone synthase====
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We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26#Streaking_of_colonies_transformed_by_BBa_K762100.2BpGEMTeasy_.28LS.29 26 August]We do PCR of all the clones:
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We use the [https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_bacterial_culture Protocol - PCR for bacterial culture] and we use the same PCR condition than on the [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#PCR August 8] but with the appropriate Primer and Biobrick.
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====PPS5====
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We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase)
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So we do a PCR in the same condition than [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/20#CAD_PCR August 20]
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====PPS3 and PPS4====
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We need to work on this plasmid tomorrow so we do some culture from the stock
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==Photo of the Day==
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[[File:Paris Saclay 1_september.jpg|600px|center]]
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 09:13, 14 October 2014

Contents

Monday 1st September

Lab Work

Construction of the fusion protein

'by Hoang Vu'

We picked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent pick out.

Lemon scent

by melanie

Limone synthase

We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from 26 AugustWe do PCR of all the clones:

We use the Protocol - PCR for bacterial culture and we use the same PCR condition than on the August 8 but with the appropriate Primer and Biobrick.


PPS5

We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) So we do a PCR in the same condition than August 20


PPS3 and PPS4

We need to work on this plasmid tomorrow so we do some culture from the stock

Photo of the Day

Paris Saclay 1 september.jpg

Back to the calendar