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Team:Paris Saclay/Notebook/July/4
From 2014.igem.org
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Liquid cultures at 37°C: | Liquid cultures at 37°C: | ||
| - | *2 colonies of MG1655 | + | *2 colonies of MG1655 from 100µl dilution |
| - | *1 colony of MG1655 | + | *1 colony of MG1655 from 50µl dilution |
In a tube, add 1 colony of MG1655Z1 in 5ml LB medium, leave at 37°C during the weekend. | In a tube, add 1 colony of MG1655Z1 in 5ml LB medium, leave at 37°C during the weekend. | ||
Revision as of 12:59, 26 August 2014
Contents |
Friday 4th July
Lab Work
A - The chassis coli Odor free
Liquid cultures of MG1655 and MG1655Z1
The transduction correctly worked. Here the results:
| Dilution for 100µl phage | MG1655 (colonies grown) | MG1655Z1 (colonies grown) |
|---|---|---|
|
100µl |
9 |
0 |
| 10µl | 2 | 2 |
| 50µl | 4 | 0 |
| 0µl | 0 | 0 |
Liquid cultures at 37°C:
- 2 colonies of MG1655 from 100µl dilution
- 1 colony of MG1655 from 50µl dilution
In a tube, add 1 colony of MG1655Z1 in 5ml LB medium, leave at 37°C during the weekend.
Wiki Progress
Wiki's notebook set up by Fabio.
Human Pratices
English Day
To celebrate the 4th of July our team spoke in English all day.
Members present:
- Instructors : Alice & Solenne.
- Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
