Team:Paris Saclay/Notebook/July/31

From 2014.igem.org

(Difference between revisions)
m (Transformation of competent E.coli cells)
Line 1: Line 1:
 +
{{Team:Paris_Saclay/notebook_header}}
=Thursday 31st July=
=Thursday 31st July=
-
 
==Lab work==
==Lab work==
-
 
===C - Salicylate Inducible Suppressing System===
===C - Salicylate Inducible Suppressing System===
====Transformation of competent E.coli cells====
====Transformation of competent E.coli cells====
Line 10: Line 9:
'''Samples:'''
'''Samples:'''
-
 
# Sample 2 μl
# Sample 2 μl
# Sample 1 μl
# Sample 1 μl
# DH5α Control
# DH5α Control
-
 
'''Results on 1st August morning:'''
'''Results on 1st August morning:'''
-
 
# Success, grown very well (+- 100 colonies)
# Success, grown very well (+- 100 colonies)
# Success, grown well (+- 50 colonies)
# Success, grown well (+- 50 colonies)
# Success, nothing has grown
# Success, nothing has grown
-
 
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
Line 39: Line 34:
* Students: Arnaud, Fabio, Romain, Sean and Terry.
* Students: Arnaud, Fabio, Romain, Sean and Terry.
-
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
+
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 08:30, 7 August 2014

Contents

Thursday 31st July

Lab work

C - Salicylate Inducible Suppressing System

Transformation of competent E.coli cells

by Fabio

We transformed in DH5α the Ligation's result made yesterday (BBa_J61051 with BBa_K228001).

Samples:

  1. Sample 2 μl
  2. Sample 1 μl
  3. DH5α Control

Results on 1st August morning:

  1. Success, grown very well (+- 100 colonies)
  2. Success, grown well (+- 50 colonies)
  3. Success, nothing has grown

Protocol

D - Lemon scent

by Romain

2 Precultures of DY330 with pJBEI-6409 in 2ml LB and Cm.

Human Pratices

Reunion, Handled topics:

  • Organisation of the Meeting with french teams
  • Great discussion about Human Practices
  • Discussion about the logo

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Romain, Sean and Terry.

Back to the calendar