"
Team:Paris Saclay/Notebook/July/3
From 2014.igem.org
(Difference between revisions)
(→2 - Plasmid DNA Purification) |
|||
| Line 1: | Line 1: | ||
| + | {{Team:Paris_Saclay/notebook_header}} | ||
=Thursday 3rd July= | =Thursday 3rd July= | ||
| - | |||
==Lab Work== | ==Lab Work== | ||
| - | + | ===Phages P1 transduction in MG1655 and MG1655Z1=== | |
| - | === | + | |
| - | + | ||
''by Mathias'' | ''by Mathias'' | ||
| Line 19: | Line 17: | ||
Pour in LB Kan petri dishes, incubate at 37°C. (''Turn the petri dishes over when there is no more liquid in!'') | Pour in LB Kan petri dishes, incubate at 37°C. (''Turn the petri dishes over when there is no more liquid in!'') | ||
| - | === | + | ===Plasmid DNA Purification=== |
| - | + | ||
''by Fabio, Maher, Mathieu, Marie and Romain'' | ''by Fabio, Maher, Mathieu, Marie and Romain'' | ||
| Line 35: | Line 32: | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol] | ||
| - | |||
'''Members present:''' | '''Members present:''' | ||
*Instructors : Alice & Solenne. | *Instructors : Alice & Solenne. | ||
*Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain. | *Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain. | ||
| - | + | {{Team:Paris_Saclay/notebook_footer}} | |
| - | + | ||
Revision as of 08:57, 7 August 2014
Contents |
Thursday 3rd July
Lab Work
Phages P1 transduction in MG1655 and MG1655Z1
by Mathias
In 2ml Eppendorf :
- 4 eppendorf MG1655 (0.1ml + 50µl CaCl)
- 4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)
Add 100µl of the different phages. Wait 20 minutes!
Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation
Pour in LB Kan petri dishes, incubate at 37°C. (Turn the petri dishes over when there is no more liquid in!)
Plasmid DNA Purification
by Fabio, Maher, Mathieu, Marie and Romain
- BBa_K773002 (x2)
- BBa_K517003 (x2)
- BBa_K773002 (x2)
- BBa_K592011 (x2)
- BBa_K1033910 (x2)
- BBa_K592009 (x2)
- BBa_K786002 (x2)
- BBa_K786003 (x2)
- BBa_J45014 (x2)
- BBa_I9026 (x2)
Members present:
- Instructors : Alice & Solenne.
- Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
