Team:Paris Saclay/Notebook/July/16

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(1 - Transformation in DH5α)
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{{Team:Paris_Saclay/notebook_header}}
=Wednesday 16th July=
=Wednesday 16th July=
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==Lab work==
==Lab work==
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===Transformation of competent E.coli cells===
===Transformation of competent E.coli cells===
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''by Fabio, Maher and Romain''
''by Fabio, Maher and Romain''
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'''Samples:'''
'''Samples:'''
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# Concentrated
# Concentrated
# Undiluted
# Undiluted
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'''Results:'''
'''Results:'''
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# Concentrated: 99 colonies
# Concentrated: 99 colonies
# Undiluted: 5 colonies
# Undiluted: 5 colonies
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'''Conclusion:'''
'''Conclusion:'''
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Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.
Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.
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===2 - Plasmid DNA Purification===
===2 - Plasmid DNA Purification===
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''by Mathieu''
''by Mathieu''
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==Reunion==
==Reunion==
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Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)
Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)
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''Arnaud represents us''
''Arnaud represents us''
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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==Photo of the Day==
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[[File:Paris Saclay 16_july.jpg|600px|center]]
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'''Members present:'''
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* Instructors and advisors: Alice, Solenne and Sylvie.
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* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 13:45, 14 October 2014

Contents

Wednesday 16th July

Lab work

Transformation of competent E.coli cells

by Fabio, Maher and Romain

We transformed in DH5α the following Biobrick:

  • BBa_J23119

Samples:

  1. Concentrated
  2. Undiluted
  3. Diluted 10-1
  4. Control

Results:

  1. Concentrated: 99 colonies
  2. Undiluted: 5 colonies
  3. Diluted: 0
  4. Control: 0

Conclusion: Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.

Protocol

2 - Plasmid DNA Purification

by Mathieu

  • BBa_J23100 (x2)
  • BBa_J23106 (x2)
  • BBa_J23114 (x2)

from Bacterial Culture made the 15th July

Protocol

Reunion

Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)

Reunion: Lab meeting to discuss the project. (afternoon)

Meeting with Evry's Team. (night) Arnaud represents us

Photo of the Day

Paris Saclay 16 july.jpg

Members present:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar