Team:Paris Saclay/Notebook/July/16

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{{Team:Paris_Saclay/notebook_header}}
=Wednesday 16th July=
=Wednesday 16th July=
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==Lab work==
==Lab work==
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===Transformation of competent E.coli cells===
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''by Fabio, Maher and Romain''
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We transformed in DH5α the following Biobrick:
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* BBa_J23119
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'''Samples:'''
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# Concentrated
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# Undiluted
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# Diluted 10<sup>-1</sup>
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# Control
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'''Results:'''
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# Concentrated: 99 colonies
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# Undiluted: 5 colonies
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# Diluted: 0
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# Control: 0
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'''Conclusion:'''
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Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
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===2 - Plasmid DNA Purification===
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''by Mathieu''
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*BBa_J23100 (x2)
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*BBa_J23106 (x2)
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*BBa_J23114 (x2)
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from Bacterial Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/15 15th July]
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]
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==Reunion==
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Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)
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Reunion: Lab meeting to discuss the project. (afternoon)
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Meeting with Evry's Team. (night)
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''Arnaud represents us''
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==Photo of the Day==
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[[File:Paris Saclay 16_july.jpg|600px|center]]
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'''Members present:'''
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* Instructors and advisors: Alice, Solenne and Sylvie.
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* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 13:45, 14 October 2014

Contents

Wednesday 16th July

Lab work

Transformation of competent E.coli cells

by Fabio, Maher and Romain

We transformed in DH5α the following Biobrick:

  • BBa_J23119

Samples:

  1. Concentrated
  2. Undiluted
  3. Diluted 10-1
  4. Control

Results:

  1. Concentrated: 99 colonies
  2. Undiluted: 5 colonies
  3. Diluted: 0
  4. Control: 0

Conclusion: Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.

Protocol

2 - Plasmid DNA Purification

by Mathieu

  • BBa_J23100 (x2)
  • BBa_J23106 (x2)
  • BBa_J23114 (x2)

from Bacterial Culture made the 15th July

Protocol

Reunion

Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)

Reunion: Lab meeting to discuss the project. (afternoon)

Meeting with Evry's Team. (night) Arnaud represents us

Photo of the Day

Paris Saclay 16 july.jpg

Members present:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/16"