Team:Paris Saclay/Notebook/August/7

From 2014.igem.org

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(Thursday 7th August)
(Thursday 7th August)
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'''Members present:'''
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===D - Lemon Sent===
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* Instructors and advisors: Alice.
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* Students: Eugene, Fabio, Hoang Vu, Juliette, Melanie, Pierre, Romain, Sean and Terry.
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{{Team:Paris_Saclay/notebook_footer}}
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=== Extraction of the Genomic DNA===
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''by Juliette & Terry''
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From liquid culture made the EDIT !!!!
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===D - Lemon Sent===
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin%C2%AE_Tissue Protocol]
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'''Members present:'''
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* Instructors and advisors: Alice.
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* Students: Eugene, Fabio, Hoang Vu, Juliette, Melanie, Pierre, Romain, Sean and Terry.
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{{Team:Paris_Saclay/notebook_footer}}

Revision as of 14:28, 7 August 2014

Contents

Thursday 7th August

Lab work

C - Salicylate Inducible Suppressing System

DNA extraction from gel agarose

In progress


D - Lemon Sent

Extraction of the Genomic DNA

by Juliette & Terry

From liquid culture made the EDIT !!!!

Protocol


Polymerase chain reaction

by Sean & Pierre


for this PCR, five tubes of each of the following Bio-bricks were prepared.

BBa_K517003

component volume
H2O 35.5μl
Phusion buffer 5X 10μl
dNTPs 2μl
iPS68bis 1μl
iPS69 1μl
DNA 1μl
Phusion enzyme 0.5μl


BBa_K762100


component volume
H2O 35.5μl
Phusion buffer 5X 10μl
dNTPs 2μl
iPS66 1μl
iPS67 1μl
DNA 1μl
Phusion enzyme 0.5μl

Tubes were placed in PCR machine with the following parameters.

Cycle step Temperature Time Cycle

Initial denaturation

98°C

1 min

1

Denaturation 98°C 15 s 25 - 30
Annealing 52°C 25 s 25 - 30
Extension 72°C 45 s 25-30
Final extension 72°C 10 min 1
Final extension 8°C hold 1


Members present:

  • Instructors and advisors: Alice.
  • Students: Eugene, Fabio, Hoang Vu, Juliette, Melanie, Pierre, Romain, Sean and Terry.

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