Team:Paris Saclay/Notebook/August/6

From 2014.igem.org

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==Lab work==
==Lab work==
===C - Salicylate Inducible Suppressing System===
===C - Salicylate Inducible Suppressing System===
 +
====Plasmid DNA Purification====
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''by Eugene and Fabio''
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* BBa_K1372000 Cl.1
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* BBa_K1372000 Cl.2
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From Liquid Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#C_-_Salicylate_Inducible_Suppressing_System 5th August]
 +
 +
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]
====Digestion====
====Digestion====
-
''In Progress''
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''by Eugene and Fabio''
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====Electrophoresis====
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-
''In Progress''
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In order to place the '''BBa_B0015''' just after the '''BBa_K1372000''', we will start the [http://parts.igem.org/Help:Assembly/3A_Assembly Assembly procedure] again.
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* BioBrick '''BBa_K1372000''' (Salicylate promoter + NahR + RNA suppressor) as '''Part A'''
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* BioBrick '''BBa_B0015''' (Double terminator) as '''Part B'''
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The enzymes used were:
 +
 
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* '''EcoRI''' and '''SpeI''' for '''BBa_K1372000'''
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* '''EcoRI''' and '''XbaI''' for '''BBa_B0015'''
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<font color="#F00">TODO: Illustration of the process</font>
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 +
[https://2014.igem.org/Team:Paris_Saclay/Protocols/BioBrick_Assembly#Digest_reaction BioBrick Assembly - Digest Reaction Protocol]
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====Segregate Process by Electrophoresis====
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''by Eugene and Fabio''
 +
 
 +
# BBa_K1372000 digested by '''EcoRI''' and '''SpeI'''
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# BBa_B0015 digested by '''EcoRI''' and '''XbaI'''
 +
 
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'''Results:'''
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#
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#
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''In progress''
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 +
[https://2014.igem.org/Team:Paris_Saclay/Protocols/BioBrick_Assembly#Segregate_process BioBrick Assembly - Segregate Process Protocol]
====Ligation====
====Ligation====
''In Progress''
''In Progress''

Revision as of 16:46, 6 August 2014

Contents

Wednesday 6th August

Lab work

C - Salicylate Inducible Suppressing System

Plasmid DNA Purification

by Eugene and Fabio

  • BBa_K1372000 Cl.1
  • BBa_K1372000 Cl.2

From Liquid Culture made the 5th August

Protocol

Digestion

by Eugene and Fabio

In order to place the BBa_B0015 just after the BBa_K1372000, we will start the Assembly procedure again.

  • BioBrick BBa_K1372000 (Salicylate promoter + NahR + RNA suppressor) as Part A
  • BioBrick BBa_B0015 (Double terminator) as Part B

The enzymes used were:

  • EcoRI and SpeI for BBa_K1372000
  • EcoRI and XbaI for BBa_B0015

TODO: Illustration of the process

BioBrick Assembly - Digest Reaction Protocol

Segregate Process by Electrophoresis

by Eugene and Fabio

  1. BBa_K1372000 digested by EcoRI and SpeI
  2. BBa_B0015 digested by EcoRI and XbaI

Results:

In progress

BioBrick Assembly - Segregate Process Protocol

Ligation

In Progress

Electrophoresis

In Progress

D - Lemon scent

PCR Cleanup

by Pierre & Sean

BioBricks used: BBa_K762100 and BBa_K517003 (PCR perfomed on the 4th August)

Protocol

Bibliography

By Hoang Vu

Still working on my mission: find more informations about limonene, pinene and citral A and B.


Members present:

  • Instructors and advisors: Alice.
  • Students: Eugene, Fabio, Hoang Vu, Juliette, Melanie, Pierre, Romain, Sean and Terry.

Back to the calendar

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