Friday 22nd August

Lab work

D - Lemon Scent

pPSI digestion

40µl pPSI

2µl H2O

2µL PacI

5µL FAST digest buffer

pPSI dephosphorylation

After 2 hours, add 1.5µL of Alkaline Phosphatase (AP) and let it 1 hour.

Then, inactivation of AP at 65°C during 15 minutes.

We've made a electrophoresis to check the digestion.

(photo)

Ligation of LS/GS/PS in pPSI

For each synthase, put:

1. 10 µL of synthase
2. 2 µL of pPSI
3. 2 µL of buffer
4. 5 µL of water
5. 1 µL of ligase

And let it in the freezer during 3 hours.

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