Team:Paris Saclay/Notebook/August/21

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Contents

Thursday 21st August

Lab work

D - Lemon Scent

Transformation of DH5α with CAD

by Sean

protocol

Electro elution of pPS1 digested by Pac1

by Huang vu and laetitia

We extracted the DNA from the agarose band saves yesterday

protocole of electro elution

Extraction of pPSI

By Mélanie

From preculture of bacteria with pPSI we extract the plasmid

protocol

Extraction of pGMETeasy with LS GS and PS

By Mélanie

Following the cloning made by Laeticia in pGMETeasy, i extract the plasmid (protocol)

and I do some stock of each stain


Ligation of CAD in pMCS5

by Eugene

We have made an electrophoresis of purified CAD and pMCS5.

  • Dephosphorylation of pMCS5
component volume
H2O 3 μl
pMCS5p 5 μl
10x buffer 10 μl
Fast AP 1 μl

30 min incubation in 37°c

  • 15min of inactivation 65°c
  • Ligation
component volume
CADp 10 μl
pMCS5p 2.5 μl
10x buffer 1.5 μl
Ligase 1 μl

night at 16°c


protocol

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