Team:Paris Saclay/Notebook/August/21

From 2014.igem.org

(Difference between revisions)
(D - Lemon Scent)
(Lab work)
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=Thursday 21st August=
=Thursday 21st August=
==Lab work==
==Lab work==
 +
===B - Construction of the fusion protein (color)===
 +
====Transformation of DH5α with chromoprotein====
 +
''by Sean''
 +
 +
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_e.coli_by_CaCl2 protocol]
 +
===D - Lemon Scent===
===D - Lemon Scent===
====Transformation of DH5α with CAD====
====Transformation of DH5α with CAD====

Revision as of 10:39, 22 August 2014

Contents

Thursday 21st August

Lab work

B - Construction of the fusion protein (color)

Transformation of DH5α with chromoprotein

by Sean

protocol

D - Lemon Scent

Transformation of DH5α with CAD

by Sean

protocol

Electro elution of pPS1 digested by Pac1

by Huang vu and laetitia

We extracted the DNA from the agarose band saves yesterday

protocole of electro elution

Extraction of pPSI

By Mélanie

From preculture of bacteria with pPSI we extract the plasmid

protocol

Extraction of pGMETeasy with LS GS and PS

By Mélanie

Following the cloning made by Laeticia in pGMETeasy, i extract the plasmid (protocol)

and I do some stock of each stain


Ligation of CAD in pMCS5

by Eugene

We have made an electrophoresis of purified CAD and pMCS5.

  • Dephosphorylation of pMCS5
component volume
H2O 3 μl
pMCS5p 5 μl
10x buffer 10 μl
Fast AP 1 μl

30 min incubation in 37°c

  • 15min of inactivation 65°c
  • Ligation
component volume
CADp 10 μl
pMCS5p 2.5 μl
10x buffer 1.5 μl
Ligase 1 μl

night at 16°c


protocol

Back to the calendar