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Team:Paris Saclay/Notebook/August/21
From 2014.igem.org
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"by Huang vu and laetitia" | "by Huang vu and laetitia" | ||
| - | We extracted the DNA from the agarose band saves yesterday | + | We extracted the DNA from the agarose band saves yesterday |
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| + | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Electro_extraction_of_DNA_from_agarose_gel protocole of electro elution] | ||
==== Ligation of CAD in pMCS5 ==== | ==== Ligation of CAD in pMCS5 ==== | ||
Revision as of 19:56, 21 August 2014
Contents |
Thursday 21st August
Lab work
D - Lemon Scent
Transformation of DH5α with CAD and chromoprotein
by Sean
Electro elution of pPS1 digested by Pac1
"by Huang vu and laetitia"
We extracted the DNA from the agarose band saves yesterday
Ligation of CAD in pMCS5
by Eugene
We have made an electrophoresis of purified CAD and pMCS5.
- Dephosphorylation of pMCS5
| component | volume |
|---|---|
| H2O | 3 μl |
| pMCS5p | 5 μl |
| 10x buffer | 10 μl |
| Fast AP | 1 μl |
30 min incubation in 37°c
- 15min of inactivation 65°c
- Ligation
| component | volume |
|---|---|
| CADp | 10 μl |
| pMCS5p | 2.5 μl |
| 10x buffer | 1.5 μl |
| Ligase | 1 μl |
night at 16°c
