Team:Paris Saclay/Notebook/August/19

From 2014.igem.org

(Difference between revisions)
(Lab work)
(D - Lemon Scent)
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====Purification of ligation products====
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We used the pGEMTeasy containing limonen synthase prepared previously : [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18 August 18th]
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The five samples were pooled in one and was purified using this protocol : [https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up protocol]
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Revision as of 15:15, 19 August 2014

Contents

Tuesday 19th August

Lab work

D - Lemon Scent

Digestion of PCR results

by Sean, Hoang Vu, Eugene

components volumes
PCR purified LS 15 μl
FastDigest green buffer 10X 2 μl
PacI 1 µl
H2O 2 µl
components volumes
PCR purified GS 15 μl
FastDigest green buffer 10X 2 μl
PacI 1 µl
H2O 2 µl
components volumes
PCR purified PS 15 μl
FastDigest green buffer 10X 2 μl
PacI 1 µl
H2O 2 µl


components volumes
PCR purified pPS1 30 μl
FastDigest green buffer 10X 4 μl
PacI 2 µl
H2O 4 µl


Purification of ligation products

We used the pGEMTeasy containing limonen synthase prepared previously : August 18th The five samples were pooled in one and was purified using this protocol : protocol


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