Team:Paris Saclay/Notebook/August/19

From 2014.igem.org

(Difference between revisions)
(Tuesday 19th August)
(Lab work)
Line 2: Line 2:
=Tuesday 19th August=
=Tuesday 19th August=
==Lab work==
==Lab work==
 +
 +
===D - Lemon Scent===
 +
 +
==== Digestion of PCR results  ====
 +
''by Sean, Hoang Vu, Eugene''
 +
 +
{| class="wikitable centre" width="50%"
 +
|+ 
 +
|-
 +
! scope=col | components
 +
! scope=col | volumes
 +
|-
 +
|PCR purified LS
 +
|15 μl
 +
|-
 +
|FastDigest green buffer 10X
 +
|2 μl
 +
|-
 +
|PacI
 +
|1 µl
 +
|-
 +
|H<sub>2</sub>O
 +
|2 µl
 +
|}
 +
 +
{| class="wikitable centre" width="50%"
 +
|+ 
 +
|-
 +
! scope=col | components
 +
! scope=col | volumes
 +
|-
 +
|PCR purified GS
 +
|15 μl
 +
|-
 +
|FastDigest green buffer 10X
 +
|2 μl
 +
|-
 +
|PacI
 +
|1 µl
 +
|-
 +
|H<sub>2</sub>O
 +
|2 µl
 +
|}
 +
 +
{| class="wikitable centre" width="50%"
 +
|+ 
 +
|-
 +
! scope=col | components
 +
! scope=col | volumes
 +
|-
 +
|PCR purified PS
 +
|15 μl
 +
|-
 +
|FastDigest green buffer 10X
 +
|2 μl
 +
|-
 +
|PacI
 +
|1 µl
 +
|-
 +
|H<sub>2</sub>O
 +
|2 µl
 +
|}
 +
 +
 +
{| class="wikitable centre" width="50%"
 +
|+ 
 +
|-
 +
! scope=col | components
 +
! scope=col | volumes
 +
|-
 +
|PCR purified pPS1
 +
|30 μl
 +
|-
 +
|FastDigest green buffer 10X
 +
|4 μl
 +
|-
 +
|PacI
 +
|2 µl
 +
|-
 +
|H<sub>2</sub>O
 +
|4 µl
 +
|}
 +
 +
 +
 +
{{Team:Paris_Saclay/notebook_footer}}
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 14:29, 19 August 2014

Contents

Tuesday 19th August

Lab work

D - Lemon Scent

Digestion of PCR results

by Sean, Hoang Vu, Eugene

components volumes
PCR purified LS 15 μl
FastDigest green buffer 10X 2 μl
PacI 1 µl
H2O 2 µl
components volumes
PCR purified GS 15 μl
FastDigest green buffer 10X 2 μl
PacI 1 µl
H2O 2 µl
components volumes
PCR purified PS 15 μl
FastDigest green buffer 10X 2 μl
PacI 1 µl
H2O 2 µl


components volumes
PCR purified pPS1 30 μl
FastDigest green buffer 10X 4 μl
PacI 2 µl
H2O 4 µl



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