Team:Paris Saclay/Notebook/August/13

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Contents

Wednesday 13th August

Lab work

D - Lemon scent

culture of pJBEI6409 bacteria

by Mélanie culture in LB of some clones obtain yesterday

Gel electrophoresis of BBa_K762100

by Sean

Samples used: 5µl from each tube prepared 12th August

Contents of the two tubes yielding the highest intensity were pooled.

PCR clean-up of BBa_K762100

by Sean

PCR clean-up was conducted on the the contents of the two tubes pooled earlier.

Gel electrophoresis of BBa_K762100

by Sean

Sample used: the result of the PCR clean-up conducted today.

Concentration of BBa_K762100 is very low -- the band is only slightly visible.

Cloning BBa_K517003 and p cola in pGEMTeasy

by Eugene and Sean

P cola and BBa_K517003 cloning
components volumes
PCR purified p cola/ BBa_K517003 7 μl
DreamTaq buffer 10X 1 μl
dATP 2 µl
DreamTaq 1 µl

incubate for 30 minutes at 70°C

Geraniol synthase
components volumes
2xT4ligase buffer 10 μl
pGEMTeasy 1 μl
DNA insert 4.7 µl at 16.8 ng/µl
2T4DNA ligase 1 µl
H2O 3.3 µl
Pinene synthase
components volumes
2xT4ligase buffer 10 μl
pGEMTeasy 1 μl
DNA insert 5.6 µl at 16.8 ng/µl
2T4DNA ligase 1 µl
H2O 2.4 µl

Culture of transformed bacteria

We used 3 colony of bacteria transformed with PJBEI+Apra (11/08) to lauch 3 cultures

-two cultures in 5mL LB+Apra (1/2000)

-one culture in 20mL LB+Apra (1/2000)

37°C - 24h

by Laetitia

Human Practices

Art & Design

by Terry

Unmolding yesterday's preparation. The mold was not hermetically closed, the chromoprotein is expressed in presence of oxygen.

Paris Saclay Space Cake 1.jpeg

It looks like blue tasty space cake but...

Paris Saclay Space Cake 2.jpeg

...when unmolded, you can only see a little ring of blue at 100% ( non-diluted preculture ).In fact, bacteria didn't grow.

I forgot that there are no nutrients in agar alone, bacteria won't grow without appropriate medium. I can't use LB because of its color. I must find a colorless medium.


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