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Team:Paris Saclay/Notebook/August/13
From 2014.igem.org
Contents |
Wednesday 13th August
Lab work
D - Lemon scent
Gel electrophoresis of BBa_K762100
by Sean
Cloning BBa_K517003 and p cola in pGEMTeasy
by Eugene and Sean
| components | volumes |
|---|---|
| PCR purified p cola/ BBa_K517003 | 7 μl |
| DreamTaq buffer 10X | 1 μl |
| dATP | 0.2 mM |
| DreamTaq | 5 units |
incubate for 30 minutes at 70°C
| components | volumes |
|---|---|
| 2xT4ligase buffer | 10 μl |
| pGEMTeasy | 1 μl |
| DNA insert | 4.7 µl of 16.8 ng/µl |
| 2T4DNA ligase | 1 µl |
| H2O | 3.3 µl |
| components | volumes |
|---|---|
| 2xT4ligase buffer | 10 μl |
| pGEMTeasy | 1 μl |
| DNA insert | 5.6 µl of 16.8 ng/µl |
| 2T4DNA ligase | 1 µl |
| H2O | 2.4 µl |
