Team:Paris Saclay/Notebook/August/13

From 2014.igem.org

(Difference between revisions)
(→Wednesday 13th August)
(→Cloning BBa_K517003 and p cola in pGEMTeasy)
Line 8: Line 8:
''by Sean''
''by Sean''
====Cloning BBa_K517003 and p cola in pGEMTeasy====
====Cloning BBa_K517003 and p cola in pGEMTeasy====
-
''by Eugène and Sean''
+
''by Eugene and Sean''
 +
 
 +
 
 +
{| class="wikitable centre" width="50%"
 +
|+ P cola cloning
 +
|-
 +
! scope=col | components
 +
! scope=col | volumes
 +
|-
 +
|PCR purified p cola
 +
|? μl
 +
|-
 +
|DreamTaq buffer 10X
 +
|1 μl
 +
|-
 +
|dATP
 +
|0.2 mM
 +
|-
 +
|DreamTaq
 +
|5 units
 +
|}
 +
 
 +
 
 +
{| class="wikitable centre" width="50%"
 +
|+ BBa_K517003 cloning
 +
|-
 +
! scope=col | components
 +
! scope=col | volumes
 +
|-
 +
|PCR purified BBa_K517003
 +
|? μl
 +
|-
 +
|DreamTaq buffer 10X
 +
|1 μl
 +
|-
 +
|dATP
 +
|0.2 mM
 +
|-
 +
|DreamTaq
 +
|5 units
 +
|}
{{Team:Paris_Saclay/notebook_footer}}
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 13:16, 14 August 2014

Contents

Wednesday 13th August

Lab work

D - Lemon scent

Gel electrophoresis of BBa_K762100

by Sean

Cloning BBa_K517003 and p cola in pGEMTeasy

by Eugene and Sean


P cola cloning
components volumes
PCR purified p cola ? μl
DreamTaq buffer 10X 1 μl
dATP 0.2 mM
DreamTaq 5 units


BBa_K517003 cloning
components volumes
PCR purified BBa_K517003 ? μl
DreamTaq buffer 10X 1 μl
dATP 0.2 mM
DreamTaq 5 units

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/August/13"