Team:Paris Saclay/Notebook

From 2014.igem.org

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==Planning==
==Planning==
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===A - The chassis coli Odor free===
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*Culture of the supercompetent cells MG1655 and MG1655Z1 [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
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*Phages preparation for the transduction [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July]
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*Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 [3rd July]
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*Cultures of MG1655 and MG1655Z1 [4th July]
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*Transformations test of competent cells MG1655 and MG1655Z1 [10th July]
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*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria [11th July]
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*Preparation of competent cells E. coli MG1655 and MG1655Z1 [17th July]
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*Transformation of supercompetent cells MG1655 and MG1655Z1 [18th July]
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*Results of the transformation [21st July]
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*Preparation and transformation of competent MG1655 and MG1655Z1 [22nd July]
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*PCR verification of the strains grown [25th July]
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*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 [28th July]
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*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R [29th July]
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*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 [30th July]
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*Stria of MG1655 and MG1655Z1 on LB dishes [1st August]
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*Stria of MG1655 and MG1655Z1 on LB and kan dishes [4th August]
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*PCR verification of the final strains MG1655 and MG1655Z1 [5th August]
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*Final stock of MG1655 [6th August]
===C - Salicylate Inducible Suppressing System===
===C - Salicylate Inducible Suppressing System===
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23th July]
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23th July]

Revision as of 08:49, 7 August 2014

Contents

Notebook

We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.

Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page

Planning

A - The chassis coli Odor free

  • Culture of the supercompetent cells MG1655 and MG1655Z1 30th June
  • Phages preparation for the transduction 2nd July
  • Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 [3rd July]
  • Cultures of MG1655 and MG1655Z1 [4th July]
  • Transformations test of competent cells MG1655 and MG1655Z1 [10th July]
  • Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria [11th July]
  • Preparation of competent cells E. coli MG1655 and MG1655Z1 [17th July]
  • Transformation of supercompetent cells MG1655 and MG1655Z1 [18th July]
  • Results of the transformation [21st July]
  • Preparation and transformation of competent MG1655 and MG1655Z1 [22nd July]
  • PCR verification of the strains grown [25th July]
  • Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 [28th July]
  • PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R [29th July]
  • Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 [30th July]
  • Stria of MG1655 and MG1655Z1 on LB dishes [1st August]
  • Stria of MG1655 and MG1655Z1 on LB and kan dishes [4th August]
  • PCR verification of the final strains MG1655 and MG1655Z1 [5th August]
  • Final stock of MG1655 [6th August]

C - Salicylate Inducible Suppressing System

  • Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23th July
  • Transformation of competent E.coli cells - 24th July
  • Bacterial culture - 25th July
  • Plasmid DNA Purification - 28th July
  • Digestion to check & Electrophoresis - 28th July
  • Main Digestion - 29th July
  • Segregate Process - 29th July
  • DNA extraction from gel agarose - 30th July
  • Ligation - 30th July
  • Transformation of competent E.coli cells - 31st July
  • Liquid Culture - 1st August
  • Plasmid DNA Purification - 4th August
  • Digestion to check & Electrophoresis - 4th August
  • Final Stock BBa_K1372000 - 4th August
  • Liquid Culture - 5th August
  • Plasmid DNA Purification - 6th August
  • Digestion of BBa_B0015 and BBa_K1372000 - 6th August
  • Segregate Process - 6th August
  • DNA extraction from gel agarose - 7th August
  • Ligation - 7th August
  • Transformation of competent E.coli cells - 8th August
  • Liquid Culture - 11th August
  • Plasmid DNA Purification - 12th August
  • Digestion to check & Electrophoresis - 12th August
  • Final Stock - 12th August

D - Lemon Scent

  • PCR of BBa_517003 and BBa_K762100 - 4th August
  • Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
  • PCR Cleanup of BBa_K517003 and BBa_K762100 - 6th August