Team:Paris Saclay/Notebook

From 2014.igem.org

(Difference between revisions)
(C - Salicylate Inducible Suppressing System)
m (Labwork)
 
(57 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Paris_Saclay/labwork_header}}
<html>
<html>
-
<!-- ################################# END HEADER ################################## -->
 
     <style>
     <style>
-
        #content > h1, #contentSub, #top-section #p-logo {display: none;}
 
-
        #top-section {height: auto; border: 0px none;}
 
-
        #menubar {top: 0;}
 
-
        #menubar ul {padding: 0px;}
 
-
        #menubar * {margin: 0px; background-color: #fff;color: gray;}
 
-
        #search-controls {top:70px;}
 
-
        #content { width: 100%; }
 
-
        #bodyContent .pure-menu li a {padding:5px 15px;}
 
-
        html, #globalWrapper {margin: 0px; padding: 0px; border: 0px none; background-color: #fff;}
 
-
        #content {margin-left: 0px; margin-right: 0px; padding-left: 0px; padding-right: 0px; border: 0px none;}
 
         #toc {display: none;}
         #toc {display: none;}
     </style>
     </style>
-
   
 
-
    <!-- We are using the pure css framework, inspired by Edinburgh's team, http://purecss.io/ -->
 
-
    <link rel="stylesheet" href="http://yui.yahooapis.com/pure/0.5.0/pure-min.css">
 
-
   
 
-
    <div class="pure-menu pure-menu-open pure-menu-horizontal">
 
-
        <a class="pure-menu-heading" href="https://2014.igem.org/Main_Page">
 
-
            <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px">
 
-
        </a>
 
-
 
-
        <ul>
 
-
            <li><a href="https://2014.igem.org/Team:Paris_Saclay">Home</a></li>
 
-
            <li><a href="https://2014.igem.org/Team:Paris_Saclay/Team">Team</a></li>
 
-
            <li><a href="https://igem.org/Team.cgi?year=2014&team_name=Paris_Saclay">Official Profile</a></li>
 
-
            <li><a href="https://2014.igem.org/Team:Paris_Saclay/Project">Project</a></li>
 
-
            <li><a href="https://2014.igem.org/Team:Paris_Saclay/Parts">Parts</a></li>
 
-
            <li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling">Modelling</a></li>
 
-
            <li><a href="https://2014.igem.org/Team:Paris_Saclay/Notebook">Notebook</a></li>
 
-
            <li><a href="https://2014.igem.org/Team:Paris_Saclay/Protocols">Protocols</a></li>
 
-
            <li><a href="https://2014.igem.org/Team:Paris_Saclay/Safety">Safety</a></li>
 
-
            <li><a href="https://2014.igem.org/Team:Paris_Saclay/Attributions">Attributions  </a></li>
 
-
        </ul>
 
-
    </div>
 
</html>
</html>
-
='''Notebook'''=
+
=Labwork=
-
We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.
+
==Notebook==
 +
We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.
-
[https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook&action=edit Click here to edit this page]
+
 
<html>
<html>
<link rel="stylesheet" type="text/css" href="https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/style.css&action=raw&ctype=text/css">
<link rel="stylesheet" type="text/css" href="https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/style.css&action=raw&ctype=text/css">
Line 48: Line 17:
<div id="notebook_div"></div>
<div id="notebook_div"></div>
</html>
</html>
-
==Planning==
+
{{Team:Paris_Saclay/default_footer}}
-
===C - Salicylate Inducible Suppressing System===
+
-
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23th July]
+
-
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#C_-_Salicylate_Inducible_Suppressing_System 24th July]
+
-
*Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#C_-_Salicylate_Inducible_Suppressing_System 25th July]
+
-
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July]
+
-
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July]
+
-
*Main Digestion - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July]
+
-
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July]
+
-
*DNA extraction from gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July]
+
-
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July]
+
-
*Transformation in DH5 alpha - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#C_-_Salicylate_Inducible_Suppressing_System 31st July]
+
-
*Bacterial Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#C_-_Salicylate_Inducible_Suppressing_System 31st July]
+
-
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#C_-_Salicylate_Inducible_Suppressing_System 1st August]
+
-
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August]
+
-
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August]
+
-
*Final Stock BBa_K1372000 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August]
+
-
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#C_-_Salicylate_Inducible_Suppressing_System 5th August]
+
-
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
+
-
*Digestion of BBa_B0015 and BBa_K1372000 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
+
-
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
+
-
*DNA extraction from gel agarose - ''7th August''
+
-
*Ligation - ''7th August''
+
-
*Transformation in DH5 alpha - ''8th August''
+
-
*Bacterial Culture - ''8th August''
+
-
*Liquid Culture - ''11th August''
+
-
*Plasmid DNA Purification - ''12th August''
+
-
*Digestion to check & Electrophoresis - ''12th August''
+
-
*Final Stock - ''12th August''
+
-
 
+
-
===D - Lemon Scent===
+
-
* PCR of BBa_517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Polymerase_chain_reaction 4th August]
+
-
* Gel electrophoresis of BBa_K517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#Electrophoresis 5th August]
+
-
* PCR Cleanup of BBa_K517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#PCR_Cleanup 6th August]
+

Latest revision as of 01:48, 18 October 2014

Labwork

Notebook

We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.

Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.