Team:Heidelberg/AwesomeSheet/Blots

From 2014.igem.org

Revision as of 15:10, 17 October 2014 by Jan glx (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

– Blots

ABCDEFGHIJKLMNOPQRSTUVWXYZAAABACADAEAFAGAHAI
1
TransferBIocking
2
IDGel ID
Membrane
Transfer-BufferUI/AtH20Ponceau-StainH20PictureWashSoventWashPrimaryWashSecondaryWash
3
VmA/cm^2minttt#tc
Name
t#ReagenttAntibodySolventVc#ReagenttAntibodySolventVc#Reagentt
4
minminminmin
% (m/V)
min
min
mlminmlmin
5
20601010110
6
1
7
2
8
3
9
4
10
5
11
67PVFD206060301405BSAPBS-T7203TBS-T10
mouse anti-H5
3TBS-T104TBS-T10
12
722PVFD20460
13
823PVFD
Tris-Glycin+20% MetOH
25460101040203BSATBS603
TBS-T/TBS-T/TBS
10
mouse anti-H5
3% BSA in TBS
51/1/10003
TBS-T/TBS-T/TBS
10
goat? anti mouse
3% BSA in TBS
512:204
TBS-T/TBS-T/TBS-/&TBS
10
14
93225450202103
TBS-T/TBS/TBS
3BSATBS
15
1033
16
1134
17
1231
18
1331
19
1431
20
1531
21
1649PVFD
Tris-Glycin+20% MetOH
0.04/1/4420105102103BSATBS603TBS-T10
mouse anti-H5
3% BSA in TBS
3TBS-T
goat anti-mouse
TBS-T
22
1750PVFD
Tris-Glycin+20% MetOH
0.04/1/4420105102103BSATBS603TBS-T10
mouse anti-H5
3% BSA in TBS
3TBS-T
goat anti-mouse
TBS-T
23
1851PVFD
Tris-Glycin+20% MetOH
0.04/1/4420105102103BSATBS603TBS-T10
mouse anti-H5
3% BSA in TBS
3TBS-T
goat anti-mouse
TBS-T
24
1952PVFD
Tris-Glycin+20% MetOH
4420102102103BSATBS603TBS-T10
mouse anti-H5
3% BSA in TBS
3TBS-T
goat anti-mouse
TBS-T
25
2053PVFD
Tris-Glycin+20% MetOH
4420102102103BSATBS603TBS-T10
mouse anti-H5
3% BSA in TBS
3TBS-T
goat anti-mouse
TBS-T
26
2154PVFD
Tris-Glycin+20% MetOH
4420102102103BSATBS603TBS-T10
mouse anti-H5
3% BSA in TBS
3TBS-T
goat anti-mouse
TBS-T
27
2258PVFD
Tris-Glycin+20% MetOH
2546002102103BSATBS60TBS-T10
mouse anti-H5
TBS-T
goat anti-mouse
28
23
29
24
30
25
31
26
32
27
33
28
34
29
35
30
36
31
37
32
38
33
39
34
40
35
41
36
42
37
43
38
44
39
45
40
46
41
47
42
48
43
49
44
50
45
51
46
52
47
53
48
54
49
55
50
56
51
57
52
58
53
59
54
60
55
61
56
62
57
63
58
64
59
65
60
66
61
67
62
68
63
69
64
70
65
71
66
72
67
73
68
74
69
75
70
76
71
77
72
78
73
79
74
80
75
81
76
82
77
83
78
84
79
85
80
86
81
87
82
88
83
89
84
90
85