Team:Paris Saclay/Notebook/July/11

From 2014.igem.org

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(Friday 11th July)
(Friday 11th July)
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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'''Members present:'''
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* Instructors and advisors: Alice, Solenne and Sylvie.
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* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
{{Team:Paris_Saclay/notebook_footer}}
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 14:40, 7 August 2014

Contents

Friday 11th July

Lab work

Fill in the WIKI (Notebook and Protocols)

by Juliette & Romain

A - The chassis coli Odor free

Preparation of competent cells E.coli

by Maher and Sean

Competent strains used:

  • DH5α
  • MG1655Z1

Sean did the first day, Maher did the second one.

Protocol

Electrophoresis

Electrophoresis 11.07.2014.jpg

by Fabio and Marie

  1. Cm FNR-RBS-AmilCP term (1)
  2. Cm FNR-RBS-AmilCP term (2)
  3. B0015 (1)
  4. B0015 (2)
  5. PCola-GES 10^-2 (1)
  6. PCola-GES 10^-2 (1)
  7. PCola-GES 10^-2 (2)
  8. PCola-GES 10^-2 (2)
  9. PJBG-6409 10^-2 (1)
  10. PJBG-6409 10^-2 (2)
  11. PJBG-6409 10^-2 (2)

Protocol

Members present:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

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