Team:TU Eindhoven/Design/Plasmid Design
From 2014.igem.org
Plasmid Design
The chosen vector for expression is pET29a(+). The pET29a(+) vector has a Kanamycin resistance gene. The expression of an inserted gene is induced by IPTG. In both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a(+) vector. The modified genes were first digested with the restriction enzymes and so was the pET29a(+) vector. (Figure 1 and Figure 2) After digestion the genes were ligated into the vector. For the protocol see: Vector Ligation
![](https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_PET29a_COMPx.png)
Figure 1. Plasmid design pET29a(+) COMPx
![](https://static.igem.org/mediawiki/2014/2/21/TU_Eindhoven_PET29a_COMPy.png)
Figure 2. Plasmid design pET29a(+) COMPy