Team:TU Eindhoven/Protocols


iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014


For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

Genetic Engineering Protocols

The following protocols are used in the Biolab during the modification of bacteria.

Antibody Labelling Double Transformation Plasmid Purification
Antibody Titration with FACS FACS DBCO-PEG(10kDa) Preparative Steps
Bacteria Cultering for Microfluidics FACS for sorting with DBCO-TAMRA Protein Expression
Casting and Running 15% PAGE Gel Fluorescent labelling antibodies Rolling Circle Amplification on membrane
Cell Viability Assay Overhang PCR Site Directed Mutagenesis
Colony PCR PCR Purification of DNA Fragments Vector Ligation
Creating Circular RCA Template Plasmid Gene Digestion Vector Transformation

Chemistry Protocols

The following protocols are used in the chemical synthesis processes.

SPAAC reaction monitoring with UV-Visible Spectroscopy
Labelling Amine-Modified DNA with DBCO-PEG4-NHS Ester

Microfluidics Protocols

The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.

Droplet Device Testing Oil and Water Phase (Polyacrylamide Beads)
Droplet Separation Photolithography
Oil and Water Phase (Encapsulation) Soft Lithography
iGEM Team TU Eindhoven 2014