Team:TU Eindhoven/Design/Plasmid Design

Plasmid Design

The chosen vector for expression is pET29a(+). The pET29a(+) vector has a Kanamycin resistance gene. The expression of an inserted gene is induced by IPTG. In both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a(+) vector. The modified genes were first digested with the restriction enzymes and so was the pET29a(+) vector. (Figure 1 and Figure 2) After digestion the genes were ligated into the vector. For the protocol see: Vector Ligation

Figure 1. Plasmid design pET29a(+) COMPx

Figure 2. Plasmid design pET29a(+) COMPy