Team:TU Eindhoven/Protocols

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iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Protocols

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

Genetic Engineering Protocols

The following protocols are used in the Biolab during the modification of bacteria.

Antibody Labelling FACS for sorting with DBCO-TAMRA Protein Expression Curve
Antibody Titration with FACS Overhang PCR Site Directed Mutagenesis
Bacteria Cultering for Microfluidics PCR Purification of DNA Fragments Vector Ligation
Colony PCR Plasmid Amplification Vector Transformation
Casting and Running 15% PAGE Gel Creating Circular RCA Template Plasmid Gene Digestion Plasmid Purification
FACS for sorting with DBCO-PEG(10kDa) Preparative Steps
Protein Expression Labelling antibodies with 5/6-TAMRA-NHS

Chemistry Protocols

The following protocols are used in the chemical synthesis processes.

SPAAC reaction monitoring with UV-Visible Spectroscopy
Labelling Amine-Modified DNA with DBCO-PEG4-NHS Ester

Microfluidics Protocols

The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.

Droplet Separation Oil and Water Phase (Polyacrylamide Beads)
Droplet Device Testing Photolithography
Oil and Water Phase (Encapsulation) Soft Lithography
iGEM Team TU Eindhoven 2014