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Team:TU Eindhoven/Project/Characterization/Antifouling Properties
From 2014.igem.org
Antifouling Assay
One of the applications for the click coli system was to evade the immune system. This was attempted by clicking large PEG tails to the bacteria. The PEG tails create a hydrophilic environment, blocking possible interactions between the bacteria and anti-bodies.
First PEG 10kDa was clicked to the membrane of the bacteria. The bacteria were then incubated in a solution with fluorescently labelled Anti-HA anti-bodies (316 nM), COMPx (membrane protein) contains a HA-tag which is targeted by the antibodies, and after incubation the bacteria were washed. There were two types of control groups used, positive and negative. For negative control one with only bacteria and another one with bacteria and DBCO. For the positive control bacteria with anti-HA antibody and no DBCO were used.
