Team:TU Eindhoven/Protocols

Protocols

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

Genetic Engineering Protocols

The following protocols are used in the Biolab during the modification of bacteria.

Antibody Titration with FACS	Overhang PCR	Plasmid Gene Digestion
FACS for sorting with DBCO-TAMRA	FACS for sorting with DBCO-PEG(10kDa)	Vector Ligation
Plasmid Amplification	Plasmid Purification	Preparative Steps
Vector Transformation	Site Directed Mutagenesis	Protein Expression
Protein Expression	PCR purification of DNA fragments	Protein Expression Curve

Chemistry Protocols

The following protocols are used in ...

SPAAC reaction monitoring with UV-Visible Spectroscopy

Microfluidics Protocols

The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.