Team:TU Eindhoven/Protocols

Protocols

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

Genetic Engineering Protocols

The following protocols are used in the Biolab during the modification of bacteria.

Plasmid Gene Digestion> Site Directed Mutagenesis

Antibody Titration with FACS	Overhang PCR	Protein Expression Curve
FACS for sorting with DBCO-TAMRA	FACS for sorting with DBCO-PEG(10kDa)	Vector Ligation	PCR purification of DNA fragments
Plasmid Amplification	Plasmid Purification	Preparative Steps	Protein Expression	Vector Transformation

Chemistry Protocols

SPAAC reaction monitoring with UV-Visible Spectroscopy

Microfluidics Protocols