Team:TU Eindhoven/Project/Characterization/Click Coli

From 2014.igem.org

Revision as of 19:04, 13 October 2014 by Rafiqlubken (Talk | contribs)

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Figure 1. Chemical structure of DBCO-PEG 4 -5/6-TAMRA.

Click Coli

Now that the plasmid design has been verified using the sequencing results and the SPAAC reaction has been verified using UV-VIS and LCMS, it is time to check whether the plasmids result in the wanted protein expression. To verify the expression of Clickable Outer Membrane Protein X (COMPx) and Y (COMPy), fluorescent labelled dibenzocyclooctyne (DBCO) groups, DBCO-PEG4-5/6-TAMRA (Figure 1), have been reacted with the incorporated p-azido-L-phenylalanine (pAzF).

Bibliography

iGEM Team TU Eindhoven 2014