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Team:TU Eindhoven/Design/Plasmid Design
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Plasmid Design
The chosen vector for expression is pET29a. The pET29a vector has a Kanamycin resitance gene. The expression of an inserted gene is induced by IPTG. In both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a vector. The modified genes were first digested with the restriction enzymes and so was the pET29a vector. (Figure 1 and Figure 2) After digestion the genes were ligated into the vector. For protocol see: Vector Ligation
Figure 1. Plasmid design pET29a(+) COMPx
Figure 2. Plasmid design pET29a(+) COMPy
