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Team:TU Eindhoven/Achievements/Main Results
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<ul style="color:white;margin-bottom:30px;list-style-type:disc;list-style-image:none;"> | <ul style="color:white;margin-bottom:30px;list-style-type:disc;list-style-image:none;"> | ||
<li>developed two <a href='https://2014.igem.org/Team:TU_Eindhoven/Background/Membrane_Anchors'>Clickable Outer Membrane Proteins</a>.</li> | <li>developed two <a href='https://2014.igem.org/Team:TU_Eindhoven/Background/Membrane_Anchors'>Clickable Outer Membrane Proteins</a>.</li> | ||
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<li>characterized the following properties of these proteins: | <li>characterized the following properties of these proteins: | ||
<ul style="color:white;list-style-type:disc;list-style-image:none;"> | <ul style="color:white;list-style-type:disc;list-style-image:none;"> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
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<li>improved an existing <a href='https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPy'>BioBrick (BBa_K811005)</a>.</li> | <li>improved an existing <a href='https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPy'>BioBrick (BBa_K811005)</a>.</li> | ||
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<li>delivered three new BioBricks to the registry (<a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPx">BBa_K1492000</a>, <a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPy">BBa_K1492001</a> and <a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/tRNA">BBa_K1492003</a>).</li> | <li>delivered three new BioBricks to the registry (<a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPx">BBa_K1492000</a>, <a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPy">BBa_K1492001</a> and <a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/tRNA">BBa_K1492003</a>).</li> | ||
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<li>tested two different molecular coatings on our cells: | <li>tested two different molecular coatings on our cells: | ||
<ul style="color:white;list-style-type:disc;list-style-image:none;"> | <ul style="color:white;list-style-type:disc;list-style-image:none;"> | ||
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<li><a href="https://2014.igem.org/Team:TU_Eindhoven/RCA">DNA combined with Rolling Circle Amplification</a></li> | <li><a href="https://2014.igem.org/Team:TU_Eindhoven/RCA">DNA combined with Rolling Circle Amplification</a></li> | ||
</ul></li> | </ul></li> | ||
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<li>successfully designed and developed <a href="https://2014.igem.org/Team:TU_Eindhoven/Microfluidics/Introduction">microfluidic devices:</a> | <li>successfully designed and developed <a href="https://2014.igem.org/Team:TU_Eindhoven/Microfluidics/Introduction">microfluidic devices:</a> | ||
<ul style="color:white;list-style-type:disc;list-style-image:none;"> | <ul style="color:white;list-style-type:disc;list-style-image:none;"> | ||
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<li>which allow <a href="https://2014.igem.org/Team:TU_Eindhoven/Microfluidics/Results_Cell_Encapsulation">fluorescent beads to be encapsulated.</a></li> | <li>which allow <a href="https://2014.igem.org/Team:TU_Eindhoven/Microfluidics/Results_Cell_Encapsulation">fluorescent beads to be encapsulated.</a></li> | ||
</ul></li> | </ul></li> | ||
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<li>collaborated with the Rathenau Institute in the <a href="https://2014.igem.org/Team:TU_Eindhoven/Synergene/Techno-Moral_Vignets">Synenergene</a> project.</li> | <li>collaborated with the Rathenau Institute in the <a href="https://2014.igem.org/Team:TU_Eindhoven/Synergene/Techno-Moral_Vignets">Synenergene</a> project.</li> | ||
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<li>written a <a href="https://2014.igem.org/Team:TU_Eindhoven/Society/Policy_Practices/Children's_Book">children’s book</a> used for educational purposes.</li> | <li>written a <a href="https://2014.igem.org/Team:TU_Eindhoven/Society/Policy_Practices/Children's_Book">children’s book</a> used for educational purposes.</li> | ||
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<li>undertook several outreach projects to spread knowledge on synthetic biology: | <li>undertook several outreach projects to spread knowledge on synthetic biology: | ||
<ul style="color:white;list-style-type:disc;list-style-image:none;"> | <ul style="color:white;list-style-type:disc;list-style-image:none;"> | ||
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</li> | </li> | ||
</ul></li> | </ul></li> | ||
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<li>made all our <a href="https://2014.igem.org/Team:TU_Eindhoven/Protocols">lab protocols</a> available for future teams </li> | <li>made all our <a href="https://2014.igem.org/Team:TU_Eindhoven/Protocols">lab protocols</a> available for future teams </li> | ||
</ul></p> | </ul></p> | ||
Revision as of 23:13, 17 October 2014
Main Results
Below a short point for point summary of the main results of our project is shown.
We have:
- developed two Clickable Outer Membrane Proteins.
- characterized the following properties of these proteins:
- the SPAAC click reaction on the E. coli membranes.
- the viability of E. coli cells expressing these proteins.
- improved an existing BioBrick (BBa_K811005).
- delivered three new BioBricks to the registry (BBa_K1492000, BBa_K1492001 and BBa_K1492003).
- tested two different molecular coatings on our cells:
- successfully designed and developed microfluidic devices:
- which enable the production of droplets in the desired size.
- which allow fluorescent beads to be encapsulated.
- collaborated with the Rathenau Institute in the Synenergene project.
- written a children’s book used for educational purposes.
- undertook several outreach projects to spread knowledge on synthetic biology:
- collaborated with the National Institute for Public Health (RIVM).
- held an informal information evening for those interested.
- actively reached out for press attention.
- made all our lab protocols available for future teams
