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Team:TU Eindhoven/Protocols
From 2014.igem.org
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<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/e/eb/TU_Eindhoven_Antibody_labelling.pdf">Antibody Labelling</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/e/eb/TU_Eindhoven_Antibody_labelling.pdf">Antibody Labelling</a></td> | ||
<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/0/07/TU_Eindhoven_Protocol_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/0/07/TU_Eindhoven_Protocol_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a></td> | ||
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/3/35/TU_Eindhoven_Protocol_Preparative_steps.pdf">Preparative Steps</a> </td> |
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<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/16/TU_Eindhoven_Protocol_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/16/TU_Eindhoven_Protocol_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a></td> | ||
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/c/cc/TU_Eindhoven_Protocol_Labelling_antibodies_with_56-TAMRA-NHS.pdf">Fluorescent labelling antibodies</a> </td> |
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6f/TU_Eindhoven_Protocol_Protein_expression.pdf">Protein Expression</a> </td> |
</tr> | </tr> | ||
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<td><a href="https://static.igem.org/mediawiki/2014/8/80/TU_Eindhoven_Protocol_bacteria_culturing_for_microfluidics.pdf" target="_blank">Bacteria Cultering for Microfluidics</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/8/80/TU_Eindhoven_Protocol_bacteria_culturing_for_microfluidics.pdf" target="_blank">Bacteria Cultering for Microfluidics</a></td> | ||
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_Protocol_Overhang_PCR.pdf">Overhang PCR</a> </td> |
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/8/8f/TU_Eindhoven_Protocol_Rolling_Circle_Amplification_on_cell_membrane.pdf">Rolling Circle Amplification on membrane</a> </td> |
</tr> | </tr> | ||
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| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/0/00/TU_Eindhoven_Casting_and_running_PAGE_gel.pdf">Casting and Running 15% PAGE Gel</a> </td> |
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/9/90/TU_Eindhoven_Protocol_PCR_Purification_of_Insert_Fragment.pdf">PCR Purification of DNA Fragments</a> </td> |
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/74/TU_Eindhoven_Protocol_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a> </td> |
</tr> | </tr> | ||
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<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/3/3d/TU_Eindhoven_Protocol_Colony_PCR.pdf">Colony PCR</a><a target="_blank" href="https://static.igem.org/mediawiki/2014/0/00/TU_Eindhoven_Casting_and_running_PAGE_gel.pdf"></a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/3/3d/TU_Eindhoven_Protocol_Colony_PCR.pdf">Colony PCR</a><a target="_blank" href="https://static.igem.org/mediawiki/2014/0/00/TU_Eindhoven_Casting_and_running_PAGE_gel.pdf"></a></td> | ||
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a> </td> |
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/3/35/TU_Eindhoven_Protocol_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/5/50/TU_Eindhoven_Creating_circular_RCA_template.pdf">Creating Circular RCA Template</a> </td> |
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/7d/TU_Eindhoven_Protocol_Plasmid_and_gene_digestion.pdf">Plasmid Gene Digestion</a> </td> |
<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/8/8a/TU_Eindhoven_Protocol_Transformation.pdf">Vector Transformation</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/8/8a/TU_Eindhoven_Protocol_Transformation.pdf">Vector Transformation</a></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/d/de/TU_Eindhoven_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS DBCO-PEG(10kDa)</a> </td> |
| - | <td | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/2/2b/TU_Eindhoven_Protocol_Plasmid_purification.pdf">Plasmid Purification</a> </td> |
<td> </td> | <td> </td> | ||
</tr> | </tr> | ||
Revision as of 20:48, 17 October 2014
Protocols
For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.
Genetic Engineering Protocols
The following protocols are used in the Biolab during the modification of bacteria.
Chemistry Protocols
The following protocols are used in the chemical synthesis processes.
| SPAAC reaction monitoring with UV-Visible Spectroscopy |
| Labelling Amine-Modified DNA with DBCO-PEG4-NHS Ester |
Microfluidics Protocols
The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.
| Droplet Separation | Oil and Water Phase (Polyacrylamide Beads) |
| Droplet Device Testing | Photolithography |
| Oil and Water Phase (Encapsulation) | Soft Lithography |
