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Team:TU Eindhoven/Protocols
From 2014.igem.org
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| - | <td><a | + | <td><a href="https://static.igem.org/mediawiki/2014/8/80/TU_Eindhoven_Protocol_bacteria_culturing_for_microfluidics.pdf" target="_blank">Bacteria Cultering for Microfluidics</a></td> |
<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a></td> | ||
<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a></td> | ||
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<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/68/TU_Eindhoven_Oil_and_Water_Phase_-_finished.pdf">Oil and Water Phase (Polyacrylamide Beads)</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/68/TU_Eindhoven_Oil_and_Water_Phase_-_finished.pdf">Oil and Water Phase (Polyacrylamide Beads)</a></td> | ||
Revision as of 13:19, 17 October 2014
Protocols
For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.
Genetic Engineering Protocols
The following protocols are used in the Biolab during the modification of bacteria.
Chemistry Protocols
The following protocols are used in the chemical synthesis processes.
| SPAAC reaction monitoring with UV-Visible Spectroscopy |
| Labeling Amine-Modified DNA with DBCO-PEG4-NHS Ester |
Microfluidics Protocols
The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.
| Oil and Water Phase (Polyacrylamide Beads) | |
| Droplet Separation | Photolithography |
| Droplet Device Testing | Soft Lithography |
| Oil and Water Phase (Bacterial Encapsulation) |
