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Team:TU Eindhoven/Design/Membrane Anchor Design
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<p>In short, both membrane proteins had a TAG codon introduced for the Non Natural Amino Acid and a HA-tag for detection methods. For INPNC the TAG codon and the HA-tag were introduced at the C terminus of the protein (<a href='#Fig1'>Figure 1</a>). The CPX proteins already contained a peptide library part at the N-terminus so the TAG codon was introduced in that part. This has been done by using Site Directed Mutagenesis. The HA-tag was introduced at the C-terminus (<a href='#Fig2'>Figure 2</a>). After modification the proteins were renamed to Clickable Outer Membrane Protein x (COMPx), originally CPX, and COMPy, originally INPNC.</p> | <p>In short, both membrane proteins had a TAG codon introduced for the Non Natural Amino Acid and a HA-tag for detection methods. For INPNC the TAG codon and the HA-tag were introduced at the C terminus of the protein (<a href='#Fig1'>Figure 1</a>). The CPX proteins already contained a peptide library part at the N-terminus so the TAG codon was introduced in that part. This has been done by using Site Directed Mutagenesis. The HA-tag was introduced at the C-terminus (<a href='#Fig2'>Figure 2</a>). After modification the proteins were renamed to Clickable Outer Membrane Protein x (COMPx), originally CPX, and COMPy, originally INPNC.</p> | ||
| - | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/ | + | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/7/75/TU_Eindhoven_COMPy_design.jpg" class="image_wrapper image_fr" width="1085"> |
<p style="font-size:18px;color:#CCCCCC;">Figure 1. Gene design COMPy (INPNC).</p> | <p style="font-size:18px;color:#CCCCCC;">Figure 1. Gene design COMPy (INPNC).</p> | ||
| - | <img id='Fig2' src="https://static.igem.org/mediawiki/2014/ | + | <img id='Fig2' src="https://static.igem.org/mediawiki/2014/c/cb/TU_Eindhoven_COMPx_design.jpg" class="image_wrapper image_fr" width="1085"> |
<p style="font-size:18px;color:#CCCCCC;">Figure 2. Gene design COMPy (CPX)</p> | <p style="font-size:18px;color:#CCCCCC;">Figure 2. Gene design COMPy (CPX)</p> | ||
Revision as of 14:08, 14 October 2014
Membrane Anchor Design
In short, both membrane proteins had a TAG codon introduced for the Non Natural Amino Acid and a HA-tag for detection methods. For INPNC the TAG codon and the HA-tag were introduced at the C terminus of the protein (Figure 1). The CPX proteins already contained a peptide library part at the N-terminus so the TAG codon was introduced in that part. This has been done by using Site Directed Mutagenesis. The HA-tag was introduced at the C-terminus (Figure 2). After modification the proteins were renamed to Clickable Outer Membrane Protein x (COMPx), originally CPX, and COMPy, originally INPNC.
Figure 1. Gene design COMPy (INPNC).
Figure 2. Gene design COMPy (CPX)
