Team:TU Eindhoven/Protocols
From 2014.igem.org
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- | + | <a target="_blank" href=" | |
+ | https://static.igem.org/mediawiki/2014/f/f8/Protocol_-_Protein_Expression_Curve.pdf">Protein Expression Curve</a> | ||
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- | <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a></td> | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a> |
- | + | </td> | |
- | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f0/Protocol_-_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS for sorting with DBCO-PEG(10kDa)</a></td> | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f0/Protocol_-_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS for sorting with DBCO-PEG(10kDa)</a> |
- | < | + | </td> |
- | + | <td> | |
- | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a> | |
- | + | </td> | |
- | <a target="_blank" href="https://static.igem.org/mediawiki/2014/0/0a/Protocol_-_PCR_Purification_of_Insert_Fragment.pdf">PCR purification of DNA fragments</a></ | + | <td> |
- | + | <a target="_blank" href="https://static.igem.org/mediawiki/2014/0/0a/Protocol_-_PCR_Purification_of_Insert_Fragment.pdf">PCR purification of DNA fragments</a> | |
- | + | </td> | |
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <a target="_blank" href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a> | ||
+ | </td> | ||
+ | <td> | ||
<a target="_blank" href=" | <a target="_blank" href=" | ||
- | https://static.igem.org/mediawiki/2014/2/29/Protocol_-_Plasmid_purification.pdf">Plasmid Purification</a></ | + | https://static.igem.org/mediawiki/2014/2/29/Protocol_-_Plasmid_purification.pdf">Plasmid Purification</a> |
- | + | </td> | |
+ | <td> | ||
<a target="_blank" href=" | <a target="_blank" href=" | ||
- | https://static.igem.org/mediawiki/2014/1/13/Protocol_-_Preparative_steps.pdf">Preparative Steps</a></ | + | https://static.igem.org/mediawiki/2014/1/13/Protocol_-_Preparative_steps.pdf">Preparative Steps</a> |
- | + | </td> | |
+ | <td> | ||
<a target="_blank" href=" | <a target="_blank" href=" | ||
- | + | https://static.igem.org/mediawiki/2014/4/45/Protocol_-_Protein_expression.pdf">Protein Expression</a> | |
- | + | </td> | |
- | + | ||
- | https://static.igem.org/mediawiki/2014/4/45/Protocol_-_Protein_expression.pdf">Protein Expression</a></ | + | |
<a target="_blank" href=" | <a target="_blank" href=" | ||
https://static.igem.org/mediawiki/2014/6/62/Protocol_-_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a></p> | https://static.igem.org/mediawiki/2014/6/62/Protocol_-_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a></p> | ||
- | + | <td> | |
<a target="_blank" href=" | <a target="_blank" href=" | ||
- | https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Vector Transformation</a></ | + | https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Vector Transformation</a> |
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<h3>Chemistry Protocols</h3> | <h3>Chemistry Protocols</h3> |
Revision as of 10:16, 10 October 2014
Protocols
For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.
Genetic Engineering Protocols
The following protocols are used in the Biolab during the modification of bacteria.