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Team:TU Eindhoven/Protocols
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<p>For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.</p> | <p>For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.</p> | ||
| - | < | + | <h3>Genetic Engineering Protocols</h3> |
<p>The following protocols are used in the Biolab during the modification of bacteria.</p> | <p>The following protocols are used in the Biolab during the modification of bacteria.</p> | ||
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<a target="_blank" href=" | <a target="_blank" href=" | ||
https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Vector Transformation</a></p> | https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Vector Transformation</a></p> | ||
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| + | <h3>Chemistry Protocols</h3> | ||
| + | <a href=" | ||
| + | https://static.igem.org/mediawiki/2014/3/3d/Protocol_-_Monitoring_SPAAC_with_UVVIS.pdf">SPAAC reaction monitoring with UV-Visible Spectroscopy</a> | ||
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| + | <h3>Microfluidics Protocols</h3> | ||
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</div> | </div> | ||
Revision as of 08:01, 10 October 2014
Protocols
For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.
Genetic Engineering Protocols
The following protocols are used in the Biolab during the modification of bacteria.
Antibody Titration with FACSFACS for sorting with DBCO-TAMRA
FACS for sorting with DBCO-PEG(10kDa)
Vector Ligation Overhang PCR PCR purification of DNA fragments Plasmid Amplification Plasmid Gene Digestion Plasmid Purification Preparative Steps Protein Expression Curve Protein Expression Site Directed Mutagenesis Vector Transformation
