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Team:TU Eindhoven/Design/Plasmid Design
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Revision as of 20:29, 9 October 2014
Plasmid Design
The chosen vector for expression is pET29a. At both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a vector. The modified genes were first digested with the restriction enzymes and so was the pET29a vector. (Figure 1 and Figure 2) After digestion the genes were ligated into the vector.
Figure 1. Plasmid design pET29a(+) COMPx
Figure 2. Plasmid design pET29a(+) COMPy
