Team:TU Eindhoven/Microfluidics/Droplet Device
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<h2>Droplet Device</h2> | <h2>Droplet Device</h2> | ||
- | <p>To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used ( | + | <p>To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (<a href='#Fig1'>Figure 1</a>). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow. It is possible to control the droplet size and droplet formation speed, by alternating both the oil flow as the water flow.</p> |
<img id='Fig1' src="https://static.igem.org/mediawiki/2014/c/c9/TU_Eindhoven_Droplet_Device.png" class="image_wrapper image_fr" width="1085"> | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/c/c9/TU_Eindhoven_Droplet_Device.png" class="image_wrapper image_fr" width="1085"> |
Revision as of 11:14, 9 October 2014
Droplet Device
To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (Figure 1). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow. It is possible to control the droplet size and droplet formation speed, by alternating both the oil flow as the water flow.
![](https://static.igem.org/mediawiki/2014/c/c9/TU_Eindhoven_Droplet_Device.png)
Figure 1. A droplet device with 1 oil inlet (top), 1 water inlet (middle) and an outlet (bottom). Number 1 is the filter and number 2 is the flow focusing cross junction where the droplets are formed.
Bibliography
Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91.
Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356