Team:TU Eindhoven/Microfluidics/Droplet Device

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Revision as of 11:07, 9 October 2014

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Droplet Device

To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (see Figure 1). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow. It is possible to control the droplet size and droplet formation speed, by alternating both the oil flow as the water flow.

Bibliography

Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356

Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91.

Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356

iGEM Team TU Eindhoven 2014