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Team:TU Eindhoven/Design/Plasmid Design
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<h2>Plasmid Design</h2> | <h2>Plasmid Design</h2> | ||
| - | <p>The chosen vector for expression is pET29a. The pET29a vector has a Kanamycin resitance gene. The expression of an inserted gene is induced by IPTG. In both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a vector. The modified genes were first digested with the restriction enzymes and so was the pET29a vector. (<a href='#Fig1'>Figure 1</a> and <a href='#Fig2'>Figure 2</a>) After digestion the genes were ligated into the vector. For protocol see: <a href="https:// | + | <p>The chosen vector for expression is pET29a. The pET29a vector has a Kanamycin resitance gene. The expression of an inserted gene is induced by IPTG. In both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a vector. The modified genes were first digested with the restriction enzymes and so was the pET29a vector. (<a href='#Fig1'>Figure 1</a> and <a href='#Fig2'>Figure 2</a>) After digestion the genes were ligated into the vector. For protocol see: <a href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf" target="_blank">Vector Ligation</a> </p> |
<img id='Fig1' src="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_PET29a_COMPx.png" class="image_wrapper image_fr" width="1085"> | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_PET29a_COMPx.png" class="image_wrapper image_fr" width="1085"> | ||
Revision as of 14:37, 17 October 2014
Plasmid Design
The chosen vector for expression is pET29a. The pET29a vector has a Kanamycin resitance gene. The expression of an inserted gene is induced by IPTG. In both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a vector. The modified genes were first digested with the restriction enzymes and so was the pET29a vector. (Figure 1 and Figure 2) After digestion the genes were ligated into the vector. For protocol see: Vector Ligation
Figure 1. Plasmid design pET29a(+) COMPx
Figure 2. Plasmid design pET29a(+) COMPy
