Team:TU Eindhoven/Safety/Our Lab

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                   <h2>Microfluidics: Introduction</h2>
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                   <h2>Our Lab</h2>
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                   <p>A substantial part of the TU Eindhoven iGEM 2014 Project is Microfluidics. Microfluidics is a technique that comprises various fields of engineering. This technique operates on a microscale and thus uses small volumes. For the encapsulation of bacterial cells – as is the case for our iGEM Project – droplet-based microfluidics is used. As extensively elaborated in the <a href="https://2014.igem.org/Team:TU_Eindhoven/Overview">General Overview Page</a>, the engineered bacteria must be brought in the proximity of PEG polymers. The ultimate goal is to verify the intended function of a cell encapsulation device. Theoretically three processes have to be accomplished:</p>
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                   <p>The laboratory of Chemical Biology is located in the building Helix on the TU Eindhoven campus (see <a href='#Fig1'>Figure 1</a> for a plan). This laboratory is part of the department of Biomedical Engineering. The laboratory of Chemical Biology consists of two laboratories. The first one is a ML-I laboratory with biosafety level 1 (WHO BSL 1). Most work at the ML-I lab involves recombinant expression of proteins in E. coli or yeast expression systems, and the application of bacteriophages for phage display. The second one is a ML-II laboratory (cell lab) with biosafety level 2 (WHO BSL 2). Work in the ML-II lab typically involves the use of immortalized cell lines, but it can also involve tissues or primary cells from animals or humans.</p>
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<img id='Fig1' src="https://static.igem.org/mediawiki/2014/a/ad/TU_Eindhoven_Laboratory.png" class="image_wrapper image_fr" width="1085">
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      <li>Combining a PEG solution and a bacterial culture with our engineered <i>E. coli</i> bacteria. These are the essential substances to perform the click reaction. After combining, this is called the Water Phase.</li> 
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<p style="font-size:18px;color:#CCCCCC;">Figure 1. Plan of the Laboratory of Chemical Biology.</p>
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      <li>The Water Phase has to be dispersed with an Oil Phase into droplets. This oil phase contains a fluorosurfactant in order to prevent aggregation and agglomeration of the droplets. It is crucial to form droplets with one cell in each droplet, since it will assure that each bacterial cell is encapsulated correctly – possible formation of a film is hereby avoided.</li> 
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      <li>The result of the previous two processes is a droplet with PEG and engineered E. coli bacteria inside an oil phase. With a microfluidic feature (bumpy mixer) the droplet can be stirred. The droplets are collected in a chamber where the click reaction is initiated with the use of UV light.</li> 
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Before these processes can be performed, certain questions have to be answered and certain research has to be done. For instance, how is it possible to form droplets? What is the optimal method to recollect the content of the droplets? And numerous of other questions that need answering for instance the required flow speed of the water and oil phase and the viscosities of the phases.
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<img id='Fig1' src="https://static.igem.org/mediawiki/2014/9/97/TU_Eindhoven_Microfluidics_Logo.jpg" class="image_wrapper image_fr" width="1085">
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<p style="font-size:18px;color:#CCCCCC;">Figure 1. TU Eindhoven iGEM 2014 in a microfluidic device.</p>
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<h4>Bibliography</h4>
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<p>Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
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Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91.
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Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
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Revision as of 19:47, 9 October 2014

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Our Lab

The laboratory of Chemical Biology is located in the building Helix on the TU Eindhoven campus (see Figure 1 for a plan). This laboratory is part of the department of Biomedical Engineering. The laboratory of Chemical Biology consists of two laboratories. The first one is a ML-I laboratory with biosafety level 1 (WHO BSL 1). Most work at the ML-I lab involves recombinant expression of proteins in E. coli or yeast expression systems, and the application of bacteriophages for phage display. The second one is a ML-II laboratory (cell lab) with biosafety level 2 (WHO BSL 2). Work in the ML-II lab typically involves the use of immortalized cell lines, but it can also involve tissues or primary cells from animals or humans.

Figure 1. Plan of the Laboratory of Chemical Biology.

iGEM Team TU Eindhoven 2014

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