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Team:TU Eindhoven/Microfluidics/Droplet Device
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<p>To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (<a href='#Fig1'>Figure 1</a>). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow. It is possible to control the droplet size and droplet formation speed, by alternating both the oil flow as the water flow.</p> | <p>To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (<a href='#Fig1'>Figure 1</a>). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow. It is possible to control the droplet size and droplet formation speed, by alternating both the oil flow as the water flow.</p> | ||
| - | <p>For an AutoCAD design of this microfluidic droplet device, download here. Design by Leroy Tan, Boris Arts & Rafiq Lubken.</p> | + | <p>For an AutoCAD design of this microfluidic droplet device, download <a href='#'>here</a>. Design by Leroy Tan, Boris Arts & Rafiq Lubken.</p> |
<img id='Fig1' src="https://static.igem.org/mediawiki/2014/c/c9/TU_Eindhoven_Droplet_Device.png" class="image_wrapper image_fr" width="1085"> | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/c/c9/TU_Eindhoven_Droplet_Device.png" class="image_wrapper image_fr" width="1085"> | ||
Revision as of 11:18, 9 October 2014
Droplet Device
To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (Figure 1). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow. It is possible to control the droplet size and droplet formation speed, by alternating both the oil flow as the water flow.
For an AutoCAD design of this microfluidic droplet device, download here. Design by Leroy Tan, Boris Arts & Rafiq Lubken.
Figure 1. A droplet device with 1 oil inlet (top), 1 water inlet (middle) and an outlet (bottom). Number 1 is the filter and number 2 is the flow focusing cross junction where the droplets are formed.
Bibliography
Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91.
Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
