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Team:TU Eindhoven/Microfluidics/Droplet Device
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<p>To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (<nobr><a href='#Fig3'>Figure 3</a></nobr>). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow [1]. It is possible to control the droplet size and droplet formation frequency, by alternating the rates of the oil phase and the water phase.</p> | <p>To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (<nobr><a href='#Fig3'>Figure 3</a></nobr>). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow [1]. It is possible to control the droplet size and droplet formation frequency, by alternating the rates of the oil phase and the water phase.</p> | ||
| - | <p> | + | <p>To download the AutoCAD design of this microfluidic droplet device, <a href='https://static.igem.org/mediawiki/2014/b/bf/TU_Eindhoven_Photomasks_TU_Eindhoven.zip' target="_blank">click here</a>. Design by Leroy Tan, Boris Arts & Rafiq Lubken.</p> |
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<h4>Bibliography</h4> | <h4>Bibliography</h4> | ||
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| - | [1] Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91. | + | [1] Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. <i>Nature</i>, 8(5), pp. 870-91. |
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Latest revision as of 00:19, 18 October 2014
Droplet Device
To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (
To download the AutoCAD design of this microfluidic droplet device, click here. Design by Leroy Tan, Boris Arts & Rafiq Lubken.
Figure 3. A droplet device with 1 oil inlet (top), 1 water inlet (middle) and an outlet (bottom). Number 1 is the filter and number 2 is the flow focusing cross junction where the droplets are formed.
Bibliography
[1] Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91.
