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Team:TU Eindhoven/Project/Characterization/Fluorescence Microscopy
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<h2>Fluorescence Microscopy</h2> | <h2>Fluorescence Microscopy</h2> | ||
| - | <p>In addition to the FACS results a confocal microscope was used to visualize the fluorescent cells. FACS results confirmed that the DBCO- | + | <p>In addition to the FACS results a confocal microscope was used to visualize the fluorescent cells. FACS results confirmed that the DBCO-PEG<sub>4</sub>-5/6-TAMRA molecules were clicked to the cells. The microscope assay was just an additional assay whose results could be used for presentations. In the following figures the images taken by the confocal microscope are shown. |
<br><br> | <br><br> | ||
The intensity of the pixels in the figures below gives an indication of the amount of fluorescence. The black pixel (0) means no fluorescence at all, the white (1) one represents the maximum relative fluorescence. | The intensity of the pixels in the figures below gives an indication of the amount of fluorescence. The black pixel (0) means no fluorescence at all, the white (1) one represents the maximum relative fluorescence. | ||
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<figure style="float:left;margin-left:0;margin-right:0"> | <figure style="float:left;margin-left:0;margin-right:0"> | ||
| - | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/9/99/TU_Eindhoven_FM1.jpg" width=" | + | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/9/99/TU_Eindhoven_FM1.jpg" width="320" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;"> |
<figcaption style="font-size:18px;color:#CCCCCC;">Figure 1. Image of 3 fluorescent bacteria, <br>taken with a magnification of 3.2x.</figcaption> | <figcaption style="font-size:18px;color:#CCCCCC;">Figure 1. Image of 3 fluorescent bacteria, <br>taken with a magnification of 3.2x.</figcaption> | ||
</figure> | </figure> | ||
<figure style="float:right;margin-right:0;margin-left:0"> | <figure style="float:right;margin-right:0;margin-left:0"> | ||
| - | <img id=' | + | <img id='Fig3' src="https://static.igem.org/mediawiki/2014/6/6e/TU_Eindhoven_FM2.jpg" width="320" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;"> |
| - | <figcaption style="font-size:18px;color:#CCCCCC;">Figure | + | <figcaption style="font-size:18px;color:#CCCCCC;">Figure 3. Image of the 2 fluorescent bacteria <br> seen in Figure 1. A magnification of 36.7x <br> was used.</figcaption> |
</figure> | </figure> | ||
| - | <figure style="float:right;margin-right: | + | <figure style="float:right;margin-right:5;margin-left:5"> |
| - | <img id=' | + | <img id='Fig2' src="https://static.igem.org/mediawiki/2014/9/99/TU_Eindhoven_FM3.jpg" width="320" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;"> |
| - | <figcaption style="font-size:18px;color:#CCCCCC;">Figure | + | <figcaption style="font-size:18px;color:#CCCCCC;">Figure 2. Image of the fluorescent bacterium <br> seen in figure 1 (right). A magnification of <br> 42.2x was used</figcaption> |
</figure> | </figure> | ||
Latest revision as of 11:58, 15 October 2014
Fluorescence Microscopy
In addition to the FACS results a confocal microscope was used to visualize the fluorescent cells. FACS results confirmed that the DBCO-PEG4-5/6-TAMRA molecules were clicked to the cells. The microscope assay was just an additional assay whose results could be used for presentations. In the following figures the images taken by the confocal microscope are shown.
The intensity of the pixels in the figures below gives an indication of the amount of fluorescence. The black pixel (0) means no fluorescence at all, the white (1) one represents the maximum relative fluorescence.
taken with a magnification of 3.2x.
seen in Figure 1. A magnification of 36.7x
was used.
seen in figure 1 (right). A magnification of
42.2x was used
