Team:TU Eindhoven/Protocols

From 2014.igem.org

(Difference between revisions)

Latest revision as of 00:06, 18 October 2014

iGEM Team TU Eindhoven 2014

Protocols

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

Genetic Engineering Protocols

The following protocols are used in the Biolab during the modification of bacteria.

Antibody Labelling	Double Transformation	Plasmid Purification
Antibody Titration with FACS	FACS DBCO-PEG(10kDa)	Preparative Steps
Bacteria Cultering for Microfluidics	FACS for sorting with DBCO-TAMRA	Protein Expression
Casting and Running 15% PAGE Gel	Fluorescent labelling antibodies	Rolling Circle Amplification on membrane
Cell Viability Assay	Overhang PCR	Site Directed Mutagenesis
Colony PCR	PCR Purification of DNA Fragments	Vector Ligation
Creating Circular RCA Template	Plasmid Gene Digestion	Vector Transformation

Chemistry Protocols

The following protocols are used in the chemical synthesis processes.

SPAAC reaction monitoring with UV-Visible Spectroscopy

Labelling Amine-Modified DNA with DBCO-PEG₄-NHS Ester

Microfluidics Protocols

The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.

Droplet Device Testing	Oil and Water Phase (Polyacrylamide Beads)
Droplet Separation	Photolithography
Oil and Water Phase (Encapsulation)	Soft Lithography

iGEM Team TU Eindhoven 2014

@@ Line 121: / Line 121: @@
      <div id="tooplate_wrapper">
-         <div id="contentwiki" style="height:1900px;">
+         <div id="contentwiki" style="height:1075px;">
                <div class="panel" id="home">
@@ Line 130: / Line 130: @@
                    <h3>Genetic Engineering Protocols</h3>
-<p>The following protocols are used in the Biolab during the modification of bacteria.</p>
+<p><em>The following protocols are used in the Biolab during the modification of bacteria.</em></p>
-<table border="0" style="width:1085px;">
+<table border="0" style="width:1085px;background:none;">
+  <tr>
+    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/e/eb/TU_Eindhoven_Antibody_labelling.pdf">Antibody Labelling</a></td>
+    <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/9/94/TU_Eindhoven_Protocol_Double_Transformation.pdf">Double Transformation</a> </td>
+    <td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/2/2b/TU_Eindhoven_Protocol_Plasmid_purification.pdf">Plasmid Purification</a> </td>
+  </tr>
+  <tr>
+    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/16/TU_Eindhoven_Protocol_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a></td>
+<td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/d/de/TU_Eindhoven_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS DBCO-PEG(10kDa)</a> </td>
+    <td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/3/35/TU_Eindhoven_Protocol_Preparative_steps.pdf">Preparative Steps</a> </td>
+  </tr>
+  <tr>
+    <td><a href="https://static.igem.org/mediawiki/2014/8/80/TU_Eindhoven_Protocol_bacteria_culturing_for_microfluidics.pdf" target="_blank">Bacteria Cultering for Microfluidics</a></td>
+    <td>  <a target="_blank"  href="https://static.igem.org/mediawiki/2014/0/07/TU_Eindhoven_Protocol_FACS_%28DBCO-56-TAMRA%29l.pdf">FACS for sorting with DBCO-TAMRA</a> </td>
+    <td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6f/TU_Eindhoven_Protocol_Protein_expression.pdf">Protein Expression</a> </td>
+  </tr>
+  <tr>
+    <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/0/00/TU_Eindhoven_Casting_and_running_PAGE_gel.pdf">Casting and Running 15% PAGE Gel</a> </td>
+    <td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/c/cc/TU_Eindhoven_Protocol_Labelling_antibodies_with_56-TAMRA-NHS.pdf">Fluorescent labelling antibodies</a> </td>
+    <td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/8/8f/TU_Eindhoven_Protocol_Rolling_Circle_Amplification_on_cell_membrane.pdf">Rolling Circle Amplification on membrane</a> </td>
+  </tr>
+  <tr>
+    <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6c/TU_Eindhoven_Protocol_Cell_viability.pdf">Cell Viability Assay</a> </td>
+    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_Protocol_Overhang_PCR.pdf">Overhang PCR</a><a target="_blank" href="https://static.igem.org/mediawiki/2014/9/94/TU_Eindhoven_Protocol_Double_Transformation.pdf"></a></td>
+    <td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/74/TU_Eindhoven_Protocol_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a> </td>
+  </tr>
+  <tr>
+    <td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/3/3d/TU_Eindhoven_Protocol_Colony_PCR.pdf">Colony PCR</a> </td>
+    <td>  <a target="_blank"  href="https://static.igem.org/mediawiki/2014/9/90/TU_Eindhoven_Protocol_PCR_Purification_of_Insert_Fragment.pdf">PCR Purification of DNA Fragments</a> </td>
+    <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/3/35/TU_Eindhoven_Protocol_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a> </td>
+  </tr>
    <tr>
-<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b1/Protocol_-_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a></td>
-<td>
-<a target="_blank"  href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a></td>
-<a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f0/Protocol_-_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS for sorting with DBCO-PEG(10kDa)</a>
-<br><br>
-<a target="_blank" href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a></p>
+    <td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/5/50/TU_Eindhoven_Creating_circular_RCA_template.pdf">Creating Circular RCA Template</a> </td>
+    <td>  <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/7d/TU_Eindhoven_Protocol_Plasmid_and_gene_digestion.pdf">Plasmid Gene Digestion</a> </td>
+    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/8/8a/TU_Eindhoven_Protocol_Transformation.pdf">Vector Transformation</a><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6c/TU_Eindhoven_Protocol_Cell_viability.pdf"></a></td>
+  </tr>
+</table>
-<a target="_blank" href="https://static.igem.org/mediawiki/2014/8/81/Protocol_-_Overhang_PCR.pdf">Overhang PCR</a></p>
+                  <h3>Chemistry Protocols</h3>
+<p><em>The following protocols are used in the chemical synthesis processes.</em></p>
-<a target="_blank"  href="https://static.igem.org/mediawiki/2014/0/0a/Protocol_-_PCR_Purification_of_Insert_Fragment.pdf">PCR purification of DNA fragments</a></p>
+<table border="0" style="width:1085px;background:none;">
+  <tr>
+    <td><a href="https://static.igem.org/mediawiki/2014/1/1d/TU_Eindhoven_Protocol_Monitoring_SPAAC_with_UVVIS.pdf" target="_blank">SPAAC reaction monitoring with UV-Visible Spectroscopy</a></td>
+</tr>
+<tr>
+    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/2/2f/TU_Eindhoven_Labelling_amine-modified_DNA_with_DBCO-PEG4-NHS_ester.pdf">Labelling Amine-Modified DNA with DBCO-PEG<sub>4</sub>-NHS Ester</a></td>
+  </tr>
+</table>
-<a target="_blank" href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a></p>
+                  <h3 id='Micro'>Microfluidics Protocols</h3>
-<a target="_blank" href="
+<p><em>The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.</em></p>
-https://static.igem.org/mediawiki/2014/0/07/Protocol_-_Plasmid_and_gene_digestion.pdf">Plasmid Gene Digestion</a></p>
-<a target="_blank" href="
+<table border="0" style="width:1085px;background:none;">
-https://static.igem.org/mediawiki/2014/2/29/Protocol_-_Plasmid_purification.pdf">Plasmid Purification</a></p>
+  <tr>
+    <td><a href="https://static.igem.org/mediawiki/2014/2/2e/TU_Eindhoven_Protocol_Microfluidic_Device_Testing_%28Droplets%29.pdf" target="_blank">Droplet Device Testing</a></td>
-<a target="_blank" href="
+<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/d/d5/TU_Eindhoven_Protocol_oil_and_continuous_phase.pdf">Oil and Water Phase (Polyacrylamide Beads)</a></td>
-https://static.igem.org/mediawiki/2014/1/13/Protocol_-_Preparative_steps.pdf">Preparative Steps</a></p>
+    </tr>
+<tr>
-<a target="_blank" href="
+   <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/0/09/TU_Eindhoven_Protocol_droplet_separation.pdf">Droplet Separation</a></td>
-https://static.igem.org/mediawiki/2014/f/f8/Protocol_-_Protein_Expression_Curve.pdf">Protein Expression Curve</a></p>
+    <td><a href="https://static.igem.org/mediawiki/2014/9/9f/TU_Eindhoven_Protocol_Photolithography.pdf" target="_blank">Photolithography</a></td>
+  </tr>
-<a target="_blank" href="
+<tr>
-https://static.igem.org/mediawiki/2014/4/45/Protocol_-_Protein_expression.pdf">Protein Expression</a></p>
+    <td><a href="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_Protocol_oil_and_water_phase_bead_and_bacterial_cell_encapsulation.pdf" target="_blank">Oil and Water Phase (Encapsulation)</a></td>
-<a target="_blank" href="
+        <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/8/89/TU_Eindhoven_Protocol_Soft_Lithography.pdf">Soft Lithography</a></td>
-https://static.igem.org/mediawiki/2014/6/62/Protocol_-_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a></p>
-<a target="_blank" href="
-https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Vector Transformation</a></p>
-                  <h3>Chemistry Protocols</h3>
-<a href="
-https://static.igem.org/mediawiki/2014/3/3d/Protocol_-_Monitoring_SPAAC_with_UVVIS.pdf">SPAAC reaction monitoring with UV-Visible Spectroscopy</a>
-                  <h3>Microfluidics Protocols</h3>
+  </tr>
+</table>
 </div>